An Analysis of Hypoxia- and Physioxia-Responsive Proteins and Pathways in DLBCL

A recent study provides new a new look at mechanisms by which diffuse large B-cell lymphoma (DLBCL) cells respond to a variable oxygen level. The study was published in Cells.

“Hypoxia is a common feature in most tumors, including hematological malignancies. There is a lack of studies on hypoxia- and physioxia-induced global proteome changes in lymphoma. Here, we sought to explore how the proteome of DLBCL changes when cells are exposed to acute hypoxic stress (1% of O2) and physioxia (5% of O2) for a long-time,” the researchers wrote.

This study assessed 8,239 proteins which were discerned using LC-MS/MS, of which 718, 513, and 486 had significant changes, in abundance, in the Ri-1, U2904, and U2932 cell lines.

The results showed that changes in B-NHL proteome profiles induced by hypoxia and physioxia were quantitatively similar in each cell line. However, the researchers noted, abundant proteins (DAPs) were specific to a certain cell line.

Moreover, downregulated proteins highlighted the altered cell cycle, metabolism, and interferon signaling. As expected, the researchers noted, the enrichment of upregulated proteins revealed metabolism, HIF1 signaling, and response to oxidative stress.

“Our data provide new evidence for understanding mechanisms by which DLBCL cells respond to a variable oxygen level,” the authors wrote. “Furthermore, this study reveals multiple hypoxia-responsive proteins showing an altered abundance in hypoxic and physioxic DLBCL. It remains to be investigated whether changes in the proteomes of DLBCL under normoxia and physioxia have functional consequences on lymphoma development and progression.”